We  conducted a study to decrease the cost of diagnosis and monitoring of treatment by analyse if any relationship between viral load and envelope antigen.Total number of 54 blood samples of hepatitis b positive patients who were known to be infected the time of withdrawn of blood, collected in a period of one year. The serums separated by centrifugation and preserved. All samples were tested for viral load by Thermo Fisher PCR kit and also for estimation of hepatitis b envelope antigen by indirect ELISA. Results were analysed. On the basis of this study we can conclude that there is a relationship between hepatitis b envelope antigen and viral load. When viral load reached in a patient up to a particular level antigen start rising and then both proportionally increasing. In our study that particular level of viral load >106 copies/ml. It shows if envelope antigen of hepatitis b virus present in blood, the value of viral load should be more than 106 copies/ml. In the scarcity of PCR, only Elisa for envelope antigen can draw an approximate estimation of viral load.

Rati Saxena did her MBBS in 2008 with internship in 2009 from Gajara Raja Medical College, Gwalior, India. Then MD in Microbiology from Shri Ram Murti smarak institute of medical sciences, Bareilly, India in 2017. Also have her academic career in microbiology for 7 years .  She is also certified in quality control and Antimicrobial stewardship.