Background: There are gaps in knowledge, attitude, and practice (KAP) regarding immunization and National Maternal and Child Health program among healthcare workers.

Aims and Objectives: This study aims to study the KAP gaps related to immunization among medical students at a teaching institute.

Materials and Methods: A total of 321 medical students were surveyed using a 36-item pre-validated inventory containing 36 items on knowledge, attitude, and practice with total possible scores of 55, 8, and 17, respectively. The students included all current undergraduates, interns, and postgraduates. Domain scores were analyzed for variables such as sex, age, academic year, etc., using SPSS 21.0 software. Kruskal–Wallis, Mann–Whitney U test, and Spearman correlation coefficient were employed for statistical analysis.

Results: Respondents included early MBBS students (n = 146) followed by interns (n = 106) and 1st year postgraduates (n = 69). Majority were female (55.8%), aged 22–25 years (74.8%). Mean percentage scores for knowledge and attitude were 51.41 ± 16.15% and 31.05%. For immunization subdomain of knowledge, attitude, and practice, mean scores were 45.16%, 20.32 ± 2.59%, and 36.74 ± 13.10%, respectively. The knowledge score showed a significant association with the type of student. Attitude scores did not show a significant association with demographic profile of student. Maximum scores for all domains were observed among postgraduates. Overall practice scores were much lower than knowledge and attitude. A significant correlation was observed between knowledge, attitude, and practice scores.

Conclusion: The study identifies wide gaps in KAP of medical students and the need for strategic interventions.